Identification of pro-fibrotic macrophages populations by single-cell transcriptomic analysis in West Highland white terriers affected with canine idiopathic pulmonary fibrosis.
27 февраля 2021 года
05:47
Identification of pro-fibrotic macrophages populations by single-cell transcriptomic analysis in West Highland white terriers affected with canine idiopathic pulmonary fibrosis.
Текст новости:
Title: Identification of pro-fibrotic macrophages populations by single-cell transcriptomic analysis in West Highland white terriers affected with canine idiopathic pulmonary fibrosis.
Author, co-author: Fastrès, Aline; Pirottin, Dimitri; Fievez, Laurence; Tutunaru, Alexandru-Cosmin; Bolen, Géraldine; Merveille, Anne-Christine; Marichal, Thomas; Desmet, Christophe; Bureau, Fabrice; Clercx, Cécile
Abstract: Canine idiopathic pulmonary fibrosis (CIPF) is a not well understand disease which affects old West Highland white terriers (WHWTs) and mimics idiopathic pulmonary fibrosis (IPF) in man. Recent studies in IPF using the single-cell RNA sequencing (scRNA-seq) technique revealed the presence of profibrotic macrophages populations in the lung. Here we used the scRNA-seq to characterize disease-related heterogeneity within cell subsets of macrophages/monocytes (Ma/Mo) in the BALF of 5 WHWTs affected with CIPF in comparison with 3 healthy WHWTs. Five subsets of Ma/Mo were identified. Among them, a monocytes subset present in larger proportion in CIPF WHWTs showed a gene expression profile enriched for pulmonary fibrosis processes (PFPs) (normalized enrichment score (NES) = 1.85, q-value = 0.002). Eight genes associated with PFPs were significantly overexpressed in this subset including CCL2, SPP1, FN1, CCL3, TIMP1, IL1RN, CXCL8 and CCL4. A monocytes-derived macrophages subset enriched for PFPs (NES = 1.87, q-value = 0.007) was also identified with differentially expressed genes between CIPF and healthy WHWTs. Expression in CIPF dogs in this subset was enriched for PFPs (NES = 2.01, q-value = 0.008) with significant overexpression of 4 genes associated with PFPs including FN1, SPP1, CXCL8 and PLAU. ScRNA-seq analysis of BALF specimens from healthy and CIPF WHWTs identified pro-fibrotic Ma/Mo populations enriched in pro-fibrotic genes suggesting the implication of these subpopulations in CIPF processes. Overexpressed molecules were also identified that could be used as biomarkers and/or therapeutic targets in the future.

Связанные объекты: #NES (найти в новостях), #A (найти в новостях).

Текст со страницы (автоматическое получение):
Document type :
Discipline(s) :
Life sciences : Veterinary medicine & animal health
To cite this reference:
Title :
Identification of pro-fibrotic macrophages populations by single-cell transcriptomic analysis in West Highland white terriers affected with canine idiopathic pulmonary fibrosis.
Language :
Publication date :
Keywords :
Abstract :
[en] Canine idiopathic pulmonary fibrosis (CIPF) is a not well understand disease which affects old West Highland white terriers (WHWTs) and mimics idiopathic pulmonary fibrosis (IPF) in man. Recent studies in IPF using the single-cell RNA sequencing (scRNA-seq) technique revealed the presence of profibrotic macrophages populations in the lung. Here we used the scRNA-seq to characterize disease-related heterogeneity within cell subsets of macrophages/monocytes (Ma/Mo) in the BALF of 5 WHWTs affected with CIPF in comparison with 3 healthy WHWTs. Five subsets of Ma/Mo were identified. Among them, a monocytes subset present in larger proportion in CIPF WHWTs showed a gene expression profile enriched for pulmonary fibrosis processes (PFPs) (normalized enrichment score (NES) = 1.85, q-value = 0.002). Eight genes associated with PFPs were significantly overexpressed in this subset including CCL2, SPP1, FN1, CCL3, TIMP1, IL1RN, CXCL8 and CCL4. A monocytes-derived macrophages subset enriched for PFPs (NES = 1.87, q-value = 0.007) was also identified with differentially expressed genes between CIPF and healthy WHWTs. Expression in CIPF dogs in this subset was enriched for PFPs (NES = 2.01, q-value = 0.008) with significant overexpression of 4 genes associated with PFPs including FN1, SPP1, CXCL8 and PLAU. ScRNA-seq analysis of BALF specimens from healthy and CIPF WHWTs identified pro-fibrotic Ma/Mo populations enriched in pro-fibrotic genes suggesting the implication of these subpopulations in CIPF processes. Overexpressed molecules were also identified that could be used as biomarkers and/or therapeutic targets in the future.
Funders :
Permalink :
Автоматическая система мониторинга и отбора информации
Источник
Другие материалы рубрики
★★★  26 февраля 2021 года
13:40
Cardiovascular Risk in Patients with Primary Hyperparathyroidism.
★★  26 февраля 2021 года
13:40
Vitamine D et ostéoporose
★★★★  26 февраля 2021 года
10:33
La nouvelle astronomie